RNA22 v2 microRNA target detection Share via email | Click here to view pre-computed static RNA22 v2 predictions Click here to download a program that will allow you to submit batch requests (Note: updated 09April2015, prior versions won't work) There have been 60882025 requests to date Please cite: Miranda, KC et al (2006) A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes Cell, 126, 1203-1217, 10.1016/j.cell.2006.07.031. Input up to 50 microRNA sequence(s) (fasta format): Click here for sample microRNA hsa-let-7b Input single target sequence (fasta format): Click here for sample target sequence ENSG00000169921|ENST00000304917 Output Format: Heteroduplex's Text Table Sensitivity vs. Specificity setting: Sensitivity of 92%, Specificity of 8% Sensitivity of 92%, Specificity of 9% Sensitivity of 91%, Specificity of 12% Sensitivity of 90%, Specificity of 15% Sensitivity of 89%, Specificity of 19% Sensitivity of 87%, Specificity of 25% Sensitivity of 85%, Specificity of 30% Sensitivity of 83%, Specificity of 35% Sensitivity of 80%, Specificity of 41% Sensitivity of 77%, Specificity of 45% Sensitivity of 73%, Specificity of 49% Sensitivity of 70%, Specificity of 54% Sensitivity of 67%, Specificity of 57% Sensitivity of 63%, Specificity of 61% Sensitivity of 60%, Specificity of 64% Sensitivity of 57%, Specificity of 67% Sensitivity of 54%, Specificity of 70% Sensitivity of 50%, Specificity of 73% Sensitivity of 48%, Specificity of 76% Sensitivity of 45%, Specificity of 78% Sensitivity of 42%, Specificity of 80% Sensitivity of 39%, Specificity of 81% Sensitivity of 37%, Specificity of 83% Sensitivity of 34%, Specificity of 85% Sensitivity of 32%, Specificity of 86% Sensitivity of 30%, Specificity of 87% Sensitivity of 28%, Specificity of 88% Sensitivity of 26%, Specificity of 89% Sensitivity of 25%, Specificity of 90% Sensitivity of 23%, Specificity of 91% Sensitivity of 22%, Specificity of 92% Sensitivity of 21%, Specificity of 92% Seed/nucleus region: Seed size of 6, allow maximum of 0 UN-paired bases in seed Seed size of 7, allow maximum of 0 UN-paired bases in seed Seed size of 7, allow maximum of 1 UN-paired bases in seed Seed size of 8, allow maximum of 0 UN-paired bases in seed Seed size of 8, allow maximum of 1 UN-paired bases in seed Seed size of 8, allow maximum of 2 UN-paired bases in seed Minimum number of paired-up bases in heteroduplex: 10 11 12 13 14 15 16 17 18 19 Maximum folding energy for heteroduplex (Kcal/mol): -5 -6 -7 -8 -9 -10 -11 -12 -13 -14 -15 -16 -17 -18 -19 -20 -21 -22 -23 -24 -25 -26 -27 -28 -29 -30 -31 -32 -33 -34 Maximum number of G:U wobbles allowed in seed region: No limit 0 1 2 3 4 5 6

We gratefuly acknowledge use of the Vienna RNA Package originally described in: Fast Folding and comparison of RNA secondary structures, Hofacker et al. Monatshefte fuer Chemie, Vol 125, 167-188, 1996.
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