RNA22 v2 microRNA target detection
Share via email
| Click
here
to view pre-computed static RNA22 v2 predictions
Click
here
to download a program that will allow you to submit batch requests (
Note:
updated 09April2015, prior versions won't work)
There have been
20792760
requests to date
Please cite:
Miranda, KC et al (2006)
A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes
Cell, 126, 1203-1217, 10.1016/j.cell.2006.07.031.
Input
up to 50
microRNA sequence(s) (
fasta format
):
Click here for sample microRNA
hsa-let-7b
Input
single
target sequence (
fasta format
):
Click here for sample target sequence
ENSG00000169921|ENST00000304917
Output Format:
Heteroduplex's
Text Table
Sensitivity vs. Specificity setting:
Sensitivity of 92%, Specificity of 8%
Sensitivity of 92%, Specificity of 9%
Sensitivity of 91%, Specificity of 12%
Sensitivity of 90%, Specificity of 15%
Sensitivity of 89%, Specificity of 19%
Sensitivity of 87%, Specificity of 25%
Sensitivity of 85%, Specificity of 30%
Sensitivity of 83%, Specificity of 35%
Sensitivity of 80%, Specificity of 41%
Sensitivity of 77%, Specificity of 45%
Sensitivity of 73%, Specificity of 49%
Sensitivity of 70%, Specificity of 54%
Sensitivity of 67%, Specificity of 57%
Sensitivity of 63%, Specificity of 61%
Sensitivity of 60%, Specificity of 64%
Sensitivity of 57%, Specificity of 67%
Sensitivity of 54%, Specificity of 70%
Sensitivity of 50%, Specificity of 73%
Sensitivity of 48%, Specificity of 76%
Sensitivity of 45%, Specificity of 78%
Sensitivity of 42%, Specificity of 80%
Sensitivity of 39%, Specificity of 81%
Sensitivity of 37%, Specificity of 83%
Sensitivity of 34%, Specificity of 85%
Sensitivity of 32%, Specificity of 86%
Sensitivity of 30%, Specificity of 87%
Sensitivity of 28%, Specificity of 88%
Sensitivity of 26%, Specificity of 89%
Sensitivity of 25%, Specificity of 90%
Sensitivity of 23%, Specificity of 91%
Sensitivity of 22%, Specificity of 92%
Sensitivity of 21%, Specificity of 92%
Seed/nucleus region:
Seed size of 6, allow maximum of 0 UN-paired bases in seed
Seed size of 7, allow maximum of 0 UN-paired bases in seed
Seed size of 7, allow maximum of 1 UN-paired bases in seed
Seed size of 8, allow maximum of 0 UN-paired bases in seed
Seed size of 8, allow maximum of 1 UN-paired bases in seed
Seed size of 8, allow maximum of 2 UN-paired bases in seed
Minimum number of paired-up bases in heteroduplex:
10
11
12
13
14
15
16
17
18
19
Maximum folding energy for heteroduplex (Kcal/mol):
-5
-6
-7
-8
-9
-10
-11
-12
-13
-14
-15
-16
-17
-18
-19
-20
-21
-22
-23
-24
-25
-26
-27
-28
-29
-30
-31
-32
-33
-34
Maximum number of G:U wobbles allowed in seed region:
No limit
0
1
2
3
4
5
6
We gratefuly acknowledge use of the Vienna RNA Package originally described in: Fast Folding and comparison of RNA secondary structures, Hofacker et al.
Monatshefte fuer Chemie
, Vol 125, 167-188, 1996.