Off-Spotter: tool for CRISPR/Cas design

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Description: identify ALL genomic instances for the given combination of gRNA(s), PAM, number of mismatches, and seed (Tutorial). Limit: a single 1,000 nucleotides sequence or fewer than twenty CR-separated 20-mers.

Genome: PAM: Maximum number of mismatches: Show Annotations?
Select the positions that you want fixed ("seed"):