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Please cite: Pliatsika, V, and Rigoutsos, I (2015) "Off-Spotter: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs" Biol. Direct 10(1):4

Description: identify ALL genomic instances for the given combination of gRNA(s), PAM, number of mismatches, and seed (Tutorial). Limit: a single 1,000 nucleotides sequence or fewer than twenty CR-separated 20-mers.

Genome: PAM: Maximum number of mismatches: Show Annotations?
Select the positions that you want fixed ("seed"):

We gratefully acknowledge support of this work by the William M. Keck Foundation.
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