Threshold-seq: a tool for determining the threshold in short RNA-seq datasets

We present “Threshold-seq”¹, a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule before it can be considered different from “background” in a given dataset. The proposed scheme is easy to implement and incorporate into existing pipelines.

Package	Description	Download
Threshold-seq1	R implementation of Threshold-seq, a computational method of determining thresholds for short RNA-seq datasets.	Version: 1.1 (3/23/2017) Download

References

1. Magee, R, Loher, P, Londin, E, Rigoutsos, I. Threshold-seq: a tool for determining the threshold in short RNA-seq datasets. Bioinformatics. 2017;33 (13):2034-2036. doi: 10.1093/bioinformatics/btx073. PubMed PMID:28203700 PubMed Central PMC5870860.