Threshold-seq: a tool for determining the threshold in short RNA-seq datasets

We present “Threshold-seq”1, a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule before it can be considered different from “background” in a given dataset. The proposed scheme is easy to implement and incorporate into existing pipelines.

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Threshold-seq1 R implementation of Threshold-seq, a computational method of determining thresholds for short RNA-seq datasets. Version: 1.1 (3/23/2017)


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