Recent advances in NGC studies have revealed that cellular RNAs are expressed as multiple isoforms bearing complex heterogeneity in terminal sequences. We report the development of Dumbbell PCR (Db-PCR), an efficient and convenient method to distinctively quantify a specific individual RNA variant. Db-PCR provides a much-needed simple method for analyzing RNA terminal heterogeneity.
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- Honda, S, Kirino, Y (2015) Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences. Nucleic Acids Res.