Db-PCR for quantification of RNA variants

Db-PCR_big

Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences

Recent advances in NGC studies have revealed that cellular RNAs are expressed as multiple isoforms bearing complex heterogeneity in terminal sequences. We report the development of Dumbbell PCR (Db-PCR), an efficient and convenient method to distinctively quantify a specific individual RNA variant. Db-PCR provides a much-needed simple method for analyzing RNA terminal heterogeneity[1].

See also the press coverage of this article here!

 

References

[1] Honda, S, Kirino, Y (2015) Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences. Nucleic Acids Res.

Save

Comments are closed