Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. We have developed Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.
Four-Leaf clover qRT-PCR (FL-PCR)
References
- Honda, S, Shigematsu, M, Morichika, K, Telonis, AG, Kirino, Y (2015) “Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA” RNA Biol 12 (5):501-8.