FL-PCR for quantification of mature tRNAs

Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA1

Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. We have developed Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.

Four-Leaf clover qRT-PCR (FL-PCR)Four-Leaf clover qRT-PCR (FL-PCR)

References

  1. Honda, S, Shigematsu, M, Morichika, K, Telonis, AG, Kirino, Y (2015) “Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA” RNA Biol 12 (5):501-8.

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